1887
Volume 2014, Issue 1
  • E-ISSN: 2223-506X

Abstract

The objective of the present study was to evaluate the effect of an exogenous enzyme blend (Natuzyme®) on the fermentation responses of diets differing in neutral detergent fiber (NDF) concentrations, using a proposed gas production technique. Diets contained different ratios of alfalfa hay to wheat straw, to provide different NDF concentration, such as 320 (D1), 340 (D2) and 360 (D3) g/kg dry matter (DM). Two hundred fifty mg of milled and dried experimental diets in three runs and four replicates were weighed into a 125-ml serum bottle for an gas production trial. A solution of Natuzyme® was added 12 hours prior to the commencement of the incubation (96 h), to make a treatment of 1.68 (g/kg DM). Serum bottles containing substrates without enzyme were considered as the control. Gas production parameters at 96 h incubation were estimated and half time of gas production (t) was calculated. Another gas test was run according to t. All the incubations for each treatment were terminated at t, then gas and methane volumes recorded. Apparent dry matter degradability (ADMD) was assessed by centrifugation and ‘ml methane per mg dry matter apparently degraded’ was calculated. Results showed that level of alfalfa hay incorporation and elevation of NDF concentration in the diets significantly increased the fractional constant rate. Enzyme supplementation significantly increased the ADMD of the diets at t, whereas gas production and produced methane per mg of DM at t (MD) of the experimental diets, was decreased at t. Increasing the NDF content of the diet showed a significant contribution in altering the fermentation parameters at t. Enzyme addition is an appropriate way to control the methane emission and to improve the dry matter degradability in the ruminant gut.

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2014-03-01
2019-12-13
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References

  1. Giraldo LA, Tejido ML, Ranilla MJ, Ramos S, Carro MD. Influence of direct-fed fibrolytic exogenous enzymes on diet digestibility and ruminal activity in sheep fed a grass hay-based diet. J Anim Sci. 2008; 86::16171623.
    [Google Scholar]
  2. McAllister TA, Hristov AN, Beauchemin KA, Rode LM, Cheng KJ. Enzymes in ruminant diets. Enzymes in Farm Animal Nutrition. UK: CAB International 2001;:273298.
    [Google Scholar]
  3. Beauchemin KA, Morgavi DP, McAllister TA, Yang WZ, Rode LM. The use of feed enzymes in ruminant diets. Recent Advances in Animal Nutrition. Nottingham, UK: Nottingham University Press 2001;:297322.
    [Google Scholar]
  4. Beauchemin KA, Colombatto D, Morgavi DP, Yang WZ. Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. J Anim Sci. 2003; 81:2:E37E47.
    [Google Scholar]
  5. Wallace RJ, Wallace SJA, McKain N, Nsereko VL, Hartnell GF. Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass silages by mixed ruminal microorganisms in vitro . J Anim Sci. 2001; 79::19051916.
    [Google Scholar]
  6. Colombatto D, Herv'as G, Yang WZ, Beauchemin KA. Effects of enzyme supplementation of a total mixed ration on microbial fermentation in continuous culture, maintained at high and low pH. J Anim Sci. 2003; 81:10:26172627.
    [Google Scholar]
  7. Wang Y, McAllister TA, Rode LM, Beauchemin KA, Morgavi DP, Nsereko VL, Iwaasa AD, Yang W. Effects of an exogenous enzyme preparation on microbial protein synthesis, enzyme activity and attachment to feed in the rumen simulation technique (Rusitec). Br J Nutr. 2001; 85::325332.
    [Google Scholar]
  8. Giraldo LA, Ranilla MJ, Tejido ML, Carro MD. Effect of enzyme application method on in vitro rumen fermentation of tropical forages. J Anim Feed Sci. 2004; 13::6366.
    [Google Scholar]
  9. Bhat MK, Hazlewood GP. Enzymology and other characteristics of cellulases and xylanases. Enzymes in Farm Animal Nutrition. Oxon, UK: CABI Publishing 2001:p.11.
    [Google Scholar]
  10. Eun JS, Beauchemin KA, Schulze H. Use of exogenous fibrolytic enzymes to enhance in vitro fermentation of alfalfa hay and corn silage. J Dairy Sci. 2007; 90::14401451.
    [Google Scholar]
  11. Grings EE, Blummel M, Sudekum KH. Methodological considerations in using gas production techniques for estimating ruminal microbial efficiencies for silage-based diets. Anim Feed Sci Technol. 2005; 123-124::527545.
    [Google Scholar]
  12. Orskov ER, McDonald I. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J Agri Sci. 1979; 92::499503.
    [Google Scholar]
  13. Blummel M, Lebzien P. Predicting ruminal microbial efficiencies of dairy ration by in vitro techniques. Livest Prod Sci. 2001; 68::107117.
    [Google Scholar]
  14. Eun JS, Beauchemin KA. Enhancing in vitro degradation of alfalfa hay and corn silage using feed enzymes. J Dairy Sci. 2007; 90:6:28392851.
    [Google Scholar]
  15. Morgavi DP, Beauchemin KA, Nsereko VL, Rode LM, Iwaasa AD, Yang WZ, McAllister TA, Wang Y. Synergy between ruminal fibrolytic enzymes and enzymes from Trichoderma longibrachiatum. J Dairy Sci. 2000; 83::1310.
    [Google Scholar]
  16. Kung L Jr, Cohen MA, Rode LM, Treacher RJ. The effects of fibrolytic enzymes sprayed onto forages and fed in a total mixed ration to lactating dairy cows. J Dairy Sci. 2002; 85::23962402.
    [Google Scholar]
  17. Giraldo LA, Ranilla MJ, Tejido ML, Carro MD. Influence of exogenous fibrolytic enzymes and fumarate on methane production, microbial growth and fermentation in Rusitec fermenters. Br J Nutr. 2007; 98:4:753761.
    [Google Scholar]
  18. McGinn SM, Beauchemin KA, Coates T, Colombatto D. Methane emissions from beef cattle: effects of monensin, sunflower oil, enzymes, yeast, and fumaric acid. J Anim Sci. 2004; 82::33463356.
    [Google Scholar]
  19. Eun JS, Fellner V, Gumpertz ML. Methane production by mixed ruminal cultures incubated in dual-flow fermenters. J Dairy Sci. 2004; 87::112121.
    [Google Scholar]
  20. Krishnamoorthy U, Steingass H, Menke KH. Preliminary observations on the relationships between gas production and microbial protein synthesis in vitro . Arch Tierernahr. 1991; 41::521526.
    [Google Scholar]
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  • Article Type: Research Article
Keyword(s): enzyme , fermentation , in vitro gas production and methane
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