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Abstract

Abstract

Ovarian cancer is a highly metastatic disease. Epithelial ovarian cancer is the most lethal gynecologic malignancy with the majority of cases being diagnosed after the disease has become metastatic. Recent reports have shown that 25% of cancerous cells within tumors have features of cancer stem cells (CSCs). CSCs have been identified on the basis of their ability for self-renewal and to have the capacity to differentiate into cancer cells and also form tumors in animal models. EMT describes a mechanism by which cells lose their epithelial characteristics and acquire more migratory mesenchymal properties. It also seems to have a key role in the acquisition of invasive and migratory properties in many types of carcinoma cells. The aim of this study is to understand the molecular changes and signaling pathways associated with ovarian cancer cell transformation which may lead to the identification of targets for novel therapeutic interventions.

OVCAR-3 and hospicells were cultured in vitro according to standard procedure. OVCAR cells were stained using anti CD133 –prominin-1 and anti CD117-APC and sorted by FACS system as positive and negative. The supernatant of OVCAR CD117+ and CD117- were analyzed by cytokine array And 174 different membrane coupled anti-cytokines along with appropriate controls were studied. The gene expression patterns of OVCAR-3 cultured with supernatant of Hospicells (clone M16) or control cells were analysed and compared by a two-colour topic-defined microarray. The products of microarray consisted of genes related to cytokines, interleukins and growth factors. The up regulated 86 codes genes were analysed by DAVID functional annotation tool.

We demonstrated that OVCAR-3 cell line for several reasons did not appear to be monoclonal. It contains tow subpopulations: one expressing CD117 and the other not. The fact that OVCAR-3 expresses at the same time CD133 and CD117 is indicative of the stem cell nature of this cell line. The cytokines secreted by CD117+ and CD117- were not the same and indicate the different profile of these cells in cell behavior. Analysis was of OVCAR gene array after 8h incubation of Hospicells by DAVID functional annotation tool intricate gpl 130 (IL6 ST) cytokine receptor interaction (IL6, IL11, OSM, LIF, CNTF ligands) and angiogenic factors expression.

We found that OVCAR-3 is apparently a suitable model for studying ovarian cancer stem cells and Epithelial to Mesenchymal Transition in Ovarian Cancer Cells.

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/content/papers/10.5339/qproc.2012.stem.1.54
2012-02-01
2019-08-18
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http://instance.metastore.ingenta.com/content/papers/10.5339/qproc.2012.stem.1.54
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  • Received: 05 Mar 2012
  • Accepted: 05 Mar 2012
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