Background Diabetes mellitus (DM) is a chronic metabolic disorder caused by an absolute or relative lack of resistance to insulin. It is characterized by hyperglycemia and accompanied by various chronic vascular complications. One therapeutic approach for managing blood glucose-and thereby preventing or delaying the above-mentioned complications-is to decrease the catalytic activity of key enzymes involved in hydrolytic cleavage of dietary oligosaccharides such as α – amylase and α – glucosidase. Plants are used from antiquity as sources of medicament against various diseases. These properties are usually attributed to secondary metabolites which are the subject of a lot of research in this field. in particular phenolic compounds (class of naturally occurring pigments with ubiquitous distribution in plant Kingdom) which they display a remarkable biological properties [3]. Many plants are drug-design targets for the development of compounds for treatment of diabetes [1] Therefore, safer natural α - amylase inhibitors have been reported from plant sources [2] The Algerian flora, is one of the richest in the world, with its many species belonging to several botanical families of which a large percentage endemic, remains very little phytochemically as pharmacologically explored. In keeping with the general pattern of bringing one's contribution to the development of the vegetable reign as a source of natural bioactive substances we will be interested in the ethnobotanical and phytochemical study of some Algerian plants to discover a new therapeutics compounds, The expertise of our laboratory in natural substance from medicinal plants obtained by extractive phytochemistry reported the screening results for α - amylase inhibitory activity of more than 30 herbal extracts. This plant species were submitted to chemical screening, the analysis of the preceding biological targets led to the evaluation of the biological activity of the extracts of the specie Cleome arabica, to confirm and distinguish their antidiabetic activity by the inhibition of α-amylase.[4][2] A B S T R A C T The aim of this study consisted on the investigation of the antidiabetic and antioxidant activities of various extracts from Cleome Arabica collected on two seasons in the town of Laghouat in the steppe region of Algeria. The preliminary evaluation of the phytochemical composition of this extracts highlighted the presence of some chemical groups. This was confirmed by a quantitative analysis based on the determination of phenolic, flavonoid and condensed tannin content. The results of the effects of phenolic compounds on the kinetic catalyzed by the α – amylase using an in vitro model, to find a natural anti- diabetic compound from the plant indicate that the phenolic extracts from these plant have an inhibitory effect on the enzyme. The antioxidant activity test shows that our phenolic extracts exhibit good antioxidant capacity. Our study is the first report on potential inhibition of these plant extracts of digestive enzyme


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