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3 - Second Mustansiriyah International Dental Conference (MIDC 2023)
  • ISSN: 1999-7086
  • EISSN: 1999-7094

Abstract

Dental caries is one of preschool children’s significant common infectious chronic diseases. Early childhood caries (ECC) is the most prevalent chronic childhood disease worldwide. HNP 1-3 are part of the non-oxidative antimicrobial mechanisms of neutrophils and can increase innate immunity by stimulating pro-inflammatory responses. Interleukin-6 (IL-6) is a pleiotropic cytokine produced by various nonimmune and immune cells responsible for regulating many aspects of the local immune response.

This study was conducted to evaluate the salivary levels of HNP1–3 in children with dental caries and to study the effect of the HNP1–3 level on the secretion of IL-6 in this disease.

Eighty children 4-5 years old were enrolled in this study. They were divided into two groups; 40 children with ECC as the study group and 40 caries-free children as the control group who matched the study group in age and gender: oral examination and measurement of clinical parameters of caries experience determined by dmfs index. Saliva samples were taken from all subjects. ELISA assay was carried out to estimate salivary levels of HNP1–3 and IL-6.

The current study found a significant elevation (P<0.05) in mean levels of HNP1-3 and IL-6 among children with ECC compared to caries-free children. Moreover, salivary HNP1-3 level showed a significant positive correlation with IL-6. On the other hand, there was no significant correlation between HNP1-3 and dmfs, whereas there was a positive correlation with IL-6.

These findings indicated that high levels of HNP1–3 and IL-6 may play a crucial essential ECC, and a positive correlation between them confirms the effect of antimicrobial peptides on cytokine production.

© 2023 Kareem, Al-Ghurabi, licensee HBKU Press.
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2023-07-31
2024-09-11
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References

  1. Selwitz RH, Ismail AI, Pitts NB. Dental caries. The Lancet. 2007 Jan 6; 369:(9555):51-9.
     [Google Scholar]
  2. Dye BA, Hsu KL, Afful J. Prevalence and Measurement of Dental Caries in Young Children. Pediatr Dent. 2015; 37:(3):200-216.
     [Google Scholar]
  3. Kagihara LE, Niederhauser VP, Stark M. Assessment, management, and prevention of early childhood caries. Journal of the American Academy of Nurse Practitioners. 2009 Jan; 21:(1):1-0.
     [Google Scholar]
  4. Li Y, Zhang YE, Yang R, Zhang Q, Zou J, Kang D. Associations of social and behavioural factors with early childhood caries in Xiamen city in China. International journal of paediatric dentistry. 2011 Mar; 21:(2):103-11.
     [Google Scholar]
  5. Veale M, Ajwani S, Johnson M, Nash L, Patterson T, George A. The early childhood oral health program: a qualitative study of the perceptions of child and family health nurses in South Western Sydney, Australia. BMC Oral Health. 2016 Dec; 16:(1):1-0.
     [Google Scholar]
  6. Kumarihamy SL, Subasinghe LD, Jayasekara P, Kularatna SM, Palipana PD. The prevalence of Early Childhood Caries in 1-2 yrs olds in a semi-urban area of Sri Lanka. BMC research notes. 2011 Dec; 4:(1):1-6.
     [Google Scholar]
  7. Çolak H, Dülgergil ÇT, Dalli M, Hamidi MM. Early childhood caries update: A review of causes, diagnoses, and treatments. Journal of natural science, biology, and medicine. 2013 Jan; 4:(1):29.
     [Google Scholar]
  8. Prowse S, Schroth RJ, Wilson A, Edwards JM, Sarson J, Levi JA, Moffatt ME. Diversity considerations for promoting early childhood oral health: a pilot study. International journal of dentistry. 2014 Jan 30;2014.
     [Google Scholar]
  9. Smith L, Blinkhorn A, Moir R, Brown N, Blinkhorn F. An assessment of dental caries among young Aboriginal children in New South Wales, Australia: a cross-sectional study. BMC Public Health. 2015 Dec; 15::1-6.
     [Google Scholar]
  10. Lundy FT, Orr DF, Shaw C, Lamey PJ, Linden GJ. Detection of individual human neutrophil α-defensins (human neutrophil peptides 1, 2 and 3) in unfractionated gingival crevicular fluid—A MALDI-MS approach. Molecular immunology. 2005 Mar 1; 42:(5):575-9.
     [Google Scholar]
  11. Choi KY, Chow LN, Mookherjee N. Cationic host defence peptides: multifaceted role in immune modulation and inflammation. Journal of innate immunity. 2012; 4:(4):361-70.
     [Google Scholar]
  12. Al-Daragi, FZ, & Al-Ghurabi, BH. Effect of vitamin D on antimicrobial peptides levels in patients with periodontitis. International Journal of Health Sciences. 2022; 6:(S5), 8297–8305.
     [Google Scholar]
  13. Tang YQ, Selsted ME. Characterization of the disulfide motif in BNBD-12, an antimicrobial beta-defensin peptide from bovine neutrophils. Journal of Biological Chemistry. 1993 Mar 25; 268:(9):6649-53.
     [Google Scholar]
  14. Tani K, Murphy WJ, Chertov O, Salcedo R, Koh CY, Utsunomiya I, Funakoshi S, Asai O, Herrmann SH, Wang JM, Kwak LW. Defensins act as potent adjuvants that promote cellular and humoral immune responses in mice to a lymphoma idiotype and carrier antigens. International immunology. 2000 May 1; 12:(5):691-700.
     [Google Scholar]
  15. Lillard Jr JW, Boyaka PN, Chertov O, Oppenheim JJ, McGhee JR. Mechanisms for induction of acquired host immunity by neutrophil peptide defensins. Proceedings of the National Academy of Sciences. 1999 Jan 19; 96:(2):651-6.
     [Google Scholar]
  16. Al-Ghurabei BH. Evaluation of serum anti-cardiolipin antibody, hs-CRP and IL-6 levels in chronic periodontitis as possible risk factors for cardiovascular diseases. Journal of Baghdad college of dentistry. 2012; 24:(2):161-5.
     [Google Scholar]
  17. Shaker ZF, Hashem BH. Study the role of proinflammatory and anti-inflammatory cytokines in Iraqi chronic periodontitis patients. J Bagh Col Dent. 2012; 24:(1):164-9.
     [Google Scholar]
  18. Lo Giudice R, Militi A, Nicita F, Bruno G, Tamà C, Lo Giudice F, Puleio F, Calapai F, Mannucci C. Correlation between Oral Hygiene and IL-6 in Children. Dentistry Journal. 2020 Aug 11; 8:(3):91.
     [Google Scholar]
  19. Govula K, Anumula L, Swapna S, Kirubakaran R. Interleukin-6: A Potential Salivary Biomarker for Dental Caries Progression—A Cross-sectional Study. International Journal of Experimental Dental Science. 2021 Aug 11; 10:(1):8-13.
     [Google Scholar]
  20. Farges JC, Carrouel F, Keller JF, Baudouin C, Msika P, Bleicher F, Staquet MJ. Cytokine production by human odontoblast-like cells upon Toll-like receptor-2 engagement. Immunobiology. 2011 Apr 1; 216:(4):513-7.
     [Google Scholar]
  21. Nibali L, Fedele S, D’aiuto F, Donos N. Interleukin-6 in oral diseases: a review. Oral diseases. 2012 Apr; 18:(3):236-43.
     [Google Scholar]
  22. Al-Ghurabi BH. The Role of Soluble TLR-2 in the Immunopathogenesis of Gingivitis. International Medical Journal. 2021 Jun 2; 28:(1):37-9.
     [Google Scholar]
  23. Farges JC, Alliot-Licht B, Renard E, Ducret M, Gaudin A, Smith AJ, Cooper PR. Dental pulp defence and repair mechanisms in dental caries. Mediators of inflammation. 2015 Oct 11;2015.
     [Google Scholar]
  24. Navazesh M. Methods for collecting saliva. Annals of the New York Academy of Sciences. 1993 Sep; 694:(1):72-7.
     [Google Scholar]
  25. Güncü GN, Yilmaz D, Könönen E, Gürsoy UK. Salivary antimicrobial peptides in early detection of periodontitis. Frontiers in Cellular and Infection Microbiology. 2015 Dec 24; 5::99.
     [Google Scholar]
  26. Laputková G, Schwartzová V, Bánovčin J, Alexovič M, Sabo J. Salivary protein roles in oral health and as predictors of caries risk. Open life sciences. 2018 Jan 1; 13:(1):174-200.
     [Google Scholar]
  27. Khaudyer AT, Mohammed AT, Al-Ghurabi BH. Oral health condition among Kindergarten children in Relation to Salivary soluble Cluster of Differentiation 14 and Tool Like Receptor 2 (comparative study). Indian Journal of Public Health. 2019 Sep; 10:(9):1407.
     [Google Scholar]
  28. Yahya AA, Al-Haidar AH, Al-Mizraqchi AS. The Role of Salivary Cortisol and Mutans Streptococci in the Development of Early Childhood Caries. Indian Journal of Public Health Research & Development. 2019 Oct 1; 10:(10).
     [Google Scholar]
  29. Al-Ali GM, Jafar ZJ, AL-Ghurabi BH. The relation of salivary cathelicidin and beta-defensin with dental caries of schoolchildren. J Res Med Dent Sci. 2021 Apr; 9:(4):30-5.
     [Google Scholar]
  30. Salman HK and Aldhaher ZA. Mutans Streptococci Count and Salivary Histatin 5 Level in Relation to Early Childhood Caries. Medico-Legal Update. 2021 Apr 1; 21:(2).
     [Google Scholar]
  31. Ribeiro TR, Dria KJ, de Carvalho CB, Monteiro AJ, Fonteles MC, de Moraes Carvalho K, Fonteles CS. Salivary peptide profile and its association with early childhood caries. International journal of paediatric dentistry. 2013 May; 23:(3):225-34.
     [Google Scholar]
  32. Tao R, Jurevic RJ, Coulton KK, Tsutsui MT, Roberts MC, Kimball JR, Wells N, Berndt J, Dale BA. Salivary antimicrobial peptide expression and dental caries experience in children. Antimicrobial agents and chemotherapy. 2005 Sep; 49:(9):3883-8.
     [Google Scholar]
  33. Phattarataratip E, Olson B, Broffitt B, Qian F, Brogden KA, Drake DR, Levy SM, Banas JA. Streptococcus mutans strains recovered from caries-active or caries-free individuals differ in sensitivity to host antimicrobial peptides. Molecular oral microbiology. 2011 Jun; 26:(3):187-99.
     [Google Scholar]
  34. Anaya-López JL, López-Meza JE, Ochoa-Zarzosa A. Bacterial resistance to cationic antimicrobial peptides. Crit Rev Microbiol. 2013; 39:(2):180-195. doi:10.3109/1040841X.2012.699025
     [Google Scholar]
  35. Jha K, Sharma H, Vella V, Mandal NB, Pendyala SK, Khan MM, Francis M. Role of salivary physicochemical and peptide levels in dental caries among children: An original research. Journal of Pharmacy & Bioallied Sciences. 2022 Jul; 14:(Suppl 1):S292.
     [Google Scholar]
  36. Jayakaran TG, Rekha CV, Annamalai S, Baghkomeh PN. Salivary peptide human neutrophil defensin1–3 and its relationship with early childhood caries. Dental Research Journal. 2020 Nov; 17:(6):459.
     [Google Scholar]
  37. El-kwatehy WM, Youssef A. Salivary alpha defensin 1–3, total protein and total antioxidant in children with gingivitis. Int. J. Health Sci. Res. 2016; 7::174-80.
     [Google Scholar]
  38. Zhao A, Blackburn C, Chin J, Srinivasan M. Soluble toll like receptor 2 (TLR-2) is increased in saliva of children with dental caries. BMC Oral Health. 2014 Dec; 14:(1):1-5.
     [Google Scholar]
  39. Seyedmajidi M, Khodadadi E, Maliji G, Zaghian M, Bijani A. Neutrophil count and level of interleukin-1β and interleukin-8 in the saliva of three to five year olds with and without dental caries. Journal of Dentistry (Tehran, Iran). 2015 Sep; 12:(9):662.
     [Google Scholar]
  40. Ramirez-De los Santos S, López-Pulido EI, Medrano-Gonzalez ID, Becerra-Ruiz JS, Alonso-Sanchez CC, Vázquez-Jiménez SI, Guerrero-Velázquez C, Guzmán-Flores JM. Alteration of cytokines in saliva of children with caries and obesity. Odontology. 2021 Jan; 109::11-7.
     [Google Scholar]
  41. Gornowicz A, Bielawska A, Bielawski K, Grabowska SZ, Wójcicka A, Zalewska M, Maciorkowska E. Pro-inflammatory cytokines in saliva of adolescents with dental caries disease. Annals of agricultural and environmental medicine. 2012; 19:(4).
     [Google Scholar]
  42. Kurtiş B, Tüter G, Serdar M, Akdemir P, Uygur C, Firatli E, Bal B. Gingival crevicular fluid levels of monocyte chemoattractant protein-1 and tumor necrosis factor-alpha in patients with chronic and aggressive periodontitis. Journal of periodontology. 2005 Nov; 76:(11):1849-55.
     [Google Scholar]
  43. Gabay C. Interleukin-6 and chronic inflammation. Arthritis Res Ther. 2006; 8: Suppl 2(Suppl 2):S3. doi:10.1186/ar1917
     [Google Scholar]
  44. Menon MM, Balagopal RV, Sajitha K, Parvathy K, Sangeetha GB, Arun XM, Sureshkumar J. Evaluation of salivary interleukin-6 in children with early childhood caries after treatment. Contemporary clinical dentistry. 2016 Apr; 7:(2):198.
     [Google Scholar]
  45. Sun E, Belanger Cr, Haney Ef And Hancock Re: Host Defense (antimicrobial) peptides. Computational And Systems Biol. 2018; 10::253–285.
     [Google Scholar]
  46. Prasad SV, Fiedoruk K, Daniluk T, Piktel E, Bucki R. Expression and function of host defense peptides at inflammation sites. International journal of molecular sciences. 2019 Dec 22; 21:(1):104.
     [Google Scholar]
/content/journals/10.5339/jemtac.2023.midc.11
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Regulatory Role of Human Neutrophil Peptides (HNP1-3) on Interleukin-6 Production in Early Childhood Caries
Journal of Emergency Medicine, Trauma and Acute Care 2023, 11 (2023); https://doi.org/10.5339/jemtac.2023.midc.11
/content/journals/10.5339/jemtac.2023.midc.11
/content/journals/10.5339/jemtac.2023.midc.11
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Keyword(s): antimicrobial peptides, Early childhood caries, HNP 1–3, IL-6 and innate immune response

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