1887
3 - Second Mustansiriyah International Dental Conference (MIDC 2023)
  • ISSN: 1999-7086
  • EISSN: 1999-7094

Abstract

Pyogenic granuloma (PG) is one of the most prevalent tumor-like lesions in the mouth, with two distinct histological variants: Non-Lobular Capillary Hemangioma (NLCH) and Lobular Capillary Hemangioma (LCH). This study aims to evaluate and compare the immunohistochemical expression of Ki-67 (proliferative activity) and CD 105 (angiogenic activity) in both types to find the conflict between the two.

Data from clinical information and microscopic findings were collected. The selection criteria, including sufficient tissue, minor bleeding and/or inflammation, and proper fixation, were used to examine 24 cases of PG (10 LCH, 14 NLCH) that performed hematoxylin and eosin (H&E) staining and immunostaining of anti-Ki-67 antibodies and anti-CD105 antibodies.

Our study showed female predilection, as 66.7% of cases were female (10 cases in LCH and 6 cases in NLCH). The mean patient age was 32.71%±15.35 years. LCH type of PG showed moderate positive expression of Ki-67 in 9 cases (64.3%); only two cases (14.3%) had a negative expression, while in 6 cases (60%) of NLCH, mild positive expression was seen, and in 4 cases (40%) it was moderate. The average number of blood vessels measured in LCH and NLCH samples treated with the anti-CD105 antibody was (72.7857±39.21479) and (47.1600±30.02855) respectively, with no significant difference between the two types of PG for both markers.

The results of the present study confirm that there are no differences between the LCH and NLCH types of PG in proliferative rate and angiogenesis activity, so both types seem to have the same biological behavior.

Loading

Article metrics loading...

/content/journals/10.5339/jemtac.2023.midc.1
2023-07-19
2024-06-24
Loading full text...

Full text loading...

/deliver/fulltext/jemtac/2023/3/jemtac.2023.midc.1.html?itemId=/content/journals/10.5339/jemtac.2023.midc.1&mimeType=html&fmt=ahah

References

  1. Neville BW, Damm DD, Allen CM, Bouquot JE. Oral and maxillofacial pathology. 2nd edPhiladelphiaWB Saunders. 2002. 447–9.
    [Google Scholar]
  2. Nakamura T. Apoptosis and expression of Bax/Bcl-2 proteins in pyogenic granuloma: A comparative study with granulation tissue and capillary hemangiomaJ Cutan Pathol.2000; 27::400–5.
    [Google Scholar]
  3. International Society for the Study of Vascular Anomalies. Classification of vascular anomalies. 2018. http://www.issva.org/UserFiles/file/ISSVA-Classification-2018.pdf. Accessed July 9, 2020
    [Google Scholar]
  4. Epivatianos A, Antoniades D, Zaraboukas T, et al. Pyogenic granuloma of the oral cavity: comparative study of its clinicopathological and immunohistochemical features.Pathol Int.2005; 55:(7)391-397.
    [Google Scholar]
  5. Rezvani G, Azarpira N, Bita G, Zeynab R. Proliferative activity in oral pyogenic granuloma: a comparative immunohistochemical study.Indian J Pathol Microbiol.2010; 53:(3)403–407.
    [Google Scholar]
  6. Ribeiro J., Moraes R., Carvalho B., Nascimento A., Milhan N., Anbinder A. Oral pyogenic granuloma: An 18-year retrospective clinicopathological and immunohistochemical study. J Cutan Pathol. 2021;1–7.
    [Google Scholar]
  7. Isaza-Guzmán D, Teller-Carrero C, Laberry-Bermúdez M, GonzálesPérez L, Tobón-Arroyave S. Assessment of clinicopathological characteristics and immunoexpression of COX-2 and IL-10 in oral pyogenic granuloma. Arch Oral Biol. 2012; 57::(5)503–512.
    [Google Scholar]
  8. Park S, Lee J, Tak M, Lee H, Choi H. A research of pyogenic granuloma genesis factor with immunohistochemical analysis. J Craniofac Surg. 2017; 28:(8):2068–2072.
    [Google Scholar]
  9. Toida M, Hasegawa T, Watanabe F, et al. Lobular capillary hemangioma of the oral mucosa: clinicopathological study of 43 cases with a special reference to immunohistochemical characterization of the vascular elements. Pathol Int. 2003; 53:(1)1–7.
    [Google Scholar]
  10. Kawachi N. A comparative histological and immunohistochemical study of capillary hemangioma, pyogenic granuloma and cavernous hemangioma in oral region with special reference to vascular proliferation factor. Int. J. Oral. Med. Sci. 2011; 9:(3):241–251.
    [Google Scholar]
  11. Netto GC, Bleil CB, Hilbig A, Coutinho LM. Immunohistochemical evaluation of the microvascular density through the expression of TGF-b (CD105/endoglin) and CD34 receptors and expression of the vascular endothelial growth factor (VEGF) in oligodendrogliomas. Neuropathology. 2008; 28:(1):17–23.
    [Google Scholar]
  12. Llorca O, Trujillo A, Blanco FJ, Bernabeu C. Structural model of human endoglin, a transmembrane receptor responsible for hereditary hemorrhagic telangiectasia. J Mol Biol. 2007; 365:(3): 694–705
    [Google Scholar]
  13. Kujan O, Al-Shawaf AZ, Azzeghaiby S, AlManadille A, Aziz K, Raheel SA. Immunohistochemical comparison of p53, Ki-67, CD68, Vimentin, α-smooth muscle actin and alpha-1-antichymotrypsin in oral peripheral and central giant cell granuloma. J. Contemp. Dent Pract. 2015; 16:(1):20-24.
    [Google Scholar]
  14. Seleit IA, Asaad N, Maree A, Abdel Wahed M. Immunohistochemical expression of p53 and Ki-67 in cutaneous lupus erythematosus. J. Egypt Women Dermatol. Soc. 2010; 7:(1):5-15
    [Google Scholar]
  15. Maeda K, Chung YS, Takatsuka S, Ogawa Y, Onoda N, Sawada T, Kato Y, Nitta A, Arimoto Y, Kondo Y. Tumor angiogenesis and tumour cell proliferation as prognostic indicators in gastric carcinoma. Br J Cancer. 1995; 72:(2):319–323
    [Google Scholar]
  16. Krishnapillai R, Punnoose K, Angadi P, Koneru A. Oral pyogenic granuloma — a review of 215 cases in a south Indian teaching hospital, Karnataka, over a period of 20 years. Oral Maxillofac Surg. 2012; 16:(3): 305-309.
    [Google Scholar]
  17. Sharma S, Chandra S, Gupta S, Srivasta S. Heterogeneous conceptualization of etiopathogenesis: Oral pyogenic granuloma. Natl J Maxillofac Surg. 2019; 10:(1)3–7.
    [Google Scholar]
  18. Gordón-Núñez MA, Carvalho MV, Benevenuto TG, Lopes MFF, Silva LMM, Galvao HC. Oral pyogenic granuloma: a retrospective analysis of 293 cases in a Brazilian population. J Oral Maxillofac Surg. 2010; 68:(9)2185–2188.
    [Google Scholar]
  19. Kamal R, Dahiya P, Puri A. Oral pyogenic granuloma: various concepts of etiopathogenesis. J Oral Maxillofac Pathol. 2012; 16:(1)79–82.
    [Google Scholar]
  20. Al-Rawi N. H. Localized Reactive Hyperplastic Lesions of the gingiva: A clinico-pathological study of 636 lesions from Iraq. Mustansiria Dental Journal, 2008; 5:(2): 213–218. https://doi.org/10.32828/mdj.v5i2.530.
    [Google Scholar]
  21. Jafarzadeh H, Sanatkhani M, Mohtasham N. Oral pyogenic granuloma: a review. J Oral Sci. 2006; 48:(4)167–175.
    [Google Scholar]
  22. Kanda N, Watanabe S. Regulatory roles of sex hormones in cutaneous biology and immunology. J Dermatol Sci. 2005; 38:(1)1–7.
    [Google Scholar]
  23. Bakir WA., Yassen NY., Mohammed ZA., Latef AH., Hasoon HA. Immunohistochemical assessment of COX-2, and Ki-67 expression in gastric cancer. Iraqi Journal of Cancer and Medical Genetics. 2014; 7:(1)16–20.
    [Google Scholar]
  24. Abdulhussain MM. The immunohistochemical expression Ki-67 In Polymorphous Low-Grade Adenocarcinoma of the Salivary Glands. Mustansiria Dental Journal, 2019; 16:(1):89-95.
    [Google Scholar]
  25. Saghafi I, Amoueian S, Montazer M, Bostan R. Assessment of VEGF, CD31 and Ki-67 immunohistochemical markers in oral pyogenic granuloma: A comparison with hemangioma and inflammatory gingivitis. Iran J. Basic Med. Sci. 2011; 14:(2)185–189.
    [Google Scholar]
  26. Dyduch G, Okoρ K, Mierzyρski W. Benign vascular proliferations an immunohistochemical and comparative study. Pol J Pathol 2004 55:59–64.
    [Google Scholar]
  27. Al-Shammari, A., Allak, W., Umran, M., Yaseen, N. and Hussien, A. Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured in Vitro. Advances in Breast Cancer Research. 2015; 4:, 100-108.
    [Google Scholar]
  28. Taher JM, Abdullah BH, Hussain WA. Immunohistochemical expression of endocan, as a marker of assessment of angiogenic potential in benign vascular lesions (hemangioma, lymphangioma and lobular capillary hemangioma) of head and neck region. J Bagh coll Dent. 2018; 30:(4):61-4.
    [Google Scholar]
  29. Hameed A., Noel K., Akkila S. Placental angiogenesis, IUGR & CMV awareness in Iraqi women. Current Issues in Pharmacy and medical sciences. (2022); 35:(3):147-151.
    [Google Scholar]
  30. Vassilopoulos S., Tosios K., Panis V., Vrotsos J. Endothelial cells of oral pyogenic granulomas express eNOS and CD105 ? endoglin: an immunohistochemical study. J Oral Pathol Med. 2011; 40:: 345–351.
    [Google Scholar]
/content/journals/10.5339/jemtac.2023.midc.1
Loading
/content/journals/10.5339/jemtac.2023.midc.1
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error