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oa Effect of estradiol-17β on serum mineral concentrations of female Japanese quail
- Source: QScience Connect, Volume 2013, Issue 1, Jun 2013, 37
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- 21 August 2013
- 13 September 2013
- 01 November 2013
Abstract
Minerals have a profound impact on reproduction. The relations between estradiol-17β (E2) and blood mineral levels have been studied mainly in human and rats, but not in quail. Therefore, the aim of this study was to test the effect of E2 on serum mineral concentrations in Japanese quail (Coturnix japonica). The study was conducted at the Seljuk University animal farm thirteen week old female Japanese quails (n = 33) were housed in cages (25 × 35 × 48 cm) under a 16 hours light: 8 hours dark cycle. During the course of the study, birds were fed ad libitum with a diet supplying 20% crude protein, 2901 kcal/kg metabolic energy, 2.5% calcium (Ca), 0.35% phosphorus (P), 1.02% lysine, 1.02% methionine and cysteine mixture. After a 7-day adaptation period, the birds were randomly assigned to 3 groups, one control (n = 10) and two others as test groups (n = 11 and n = 12). Birds in test groups were subcutaneously injected with 0.1 or 0.2 mg E2. Blood samples were collected from the jugular vein and serum mineral concentrations were measured by HNO3 digestion method. Injection of 0.2 mg E2 resulted with a significant reduction in serum potassium concentration as compared to control group. Injection of 0.2 mg E2 caused a significant reduction in serum iron concentration as compared to 0.1 mg E2 injected group. Injection of 0.1 mg E2 caused a significant increase in serum chromium concentration over the control. Serum boron concentration was significantly high after the injection of 0.1 or 0.2 mg E2 over the control group. Effect of E2 on serum mineral concentration depends on injection dose. An injection of 0.1 or 0.2 mg E2 significantly increased serum boron concentration, which is an indication for the effect of E2 on bone mineralization and feed conversion ratio. Thus, administration of boron or E2 may protect postmenopausal women against osteoporosis and estradiol can be employed for the treatment of osteoporosis. Injection of 0.1 mg E2 caused a significant increase in serum chromium concentration, which indicates the function of E2 on body growth and reproductive performance. Injection of 0.2 mg E2 caused significant reduction in serum potassium (K) concentration, while there was a slight increase in serum sodium (Na) concentration. This indicates the involvement of E2 in rennin-angiotensin-aldosterone system.