1887
Volume 2012, Issue 1
  • ISSN: 0253-8253
  • E-ISSN: 2227-0426

Abstract

A study was carried out to investigate the status of p53 proteins and their relation to the occurrence of TEL-AML-1 translocation in children with acute lymphoblastic leukemia (ALL) attending the oncology unit at Basrah maternity and children hospital during the period from May 2009 to April 2010. A total of 100 blood samples were collected from 40 newly diagnosed ALL cases, and 60 healthy children served as controls. Anti-p53 antibody was detected by an enzyme-linked immunosorbent assay (ELISA), and TEL-AML-1 fusion gene was determined by reversetranscriptase polymerase reaction (RT-PCR) on RNA extracted from fresh blood samples. The overall proportion of anti-p53 was 20% in the leukemic patients, whereas none of the healthy control group showed anti-p53 positivity. This difference was statistically significant (P < 0.05). According to the French-American-British (FAB) classification, 75% of the anti-p53 positive cases were classified as L2 stage ALL. There was a significant difference (P < 0.05) between standard and high-risk groups of ALL patients in the frequency of anti-p53: 40% of high-risk group members had anti-p53 compared to 8% in the standard risk patients. There was no association (P>0.05) between TEL-AML-1 translocation and anti-p53. None of the standard risk patients with positive TEL-AML-1 fusion gene displayed the anti-p53 while 33.3% of TEL-AML-1 positive with high risk displayed the antip53 antibody.

Loading

Article metrics loading...

/content/journals/10.5339/qmj.2012.1.13
2012-06-01
2019-09-20
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.5339/qmj.2012.1.13
Loading
  • Article Type: Research Article
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error