1887
Volume 2002, Issue 2
  • ISSN: 0253-8253
  • E-ISSN: 2227-0426

Abstract

Howell-Jolly bodies (HJB) are composed ofDNA which will apparently take the fluorescent dye used to stain the chromatin bodies of the malarial parasites (MP). While evaluating a capillary tube/fluorescent staining technique for malaria we came across this previously unreported false positive effect of HJB in ten cases, all negative microscopically for MP and not originally requested for that test All gave positive results, the degree of which correlated well with the frequency of HJB. The positivity concentrated in the upper red cell layer of the capillary tube where MP rings also concentrate.

Loading

Article metrics loading...

/content/journals/10.5339/qmj.2002.2.11
2002-11-01
2019-10-21
Loading full text...

Full text loading...

References

  1. 1. Makler   MT., , Gibbins   B. . Clinics in Laboratory Medicine, Diagnosis of Important Parasitic Diseases: Laboratory Diagnosis of Malaria. . Philadelphia: : W.B. Saunders Company;   1991; ; : 941– 956 .
  2. 2. Dacie   JV., , Sir., , Lewis   SM. . Practical Hematology . , Six ed. . Singapore: : Longman Group;   1984; ; : 55– 56 .
    [Google Scholar]
  3. 3. Howard   RJ., , Aikawa   M. , et al. , Secretion of a malaria histidine-rich protein 2 (p.f.HRP-11) from Plasmodium falciparum infected erythrocytes. . J. Cell Biol.   1968; ;103: : 1269– 1277 .
    [Google Scholar]
  4. 4. Parra   ME. , et al. , Identification of Plasmodium falciparum histidine-rich protein 2 in the plasma of humans with malaria. . J. Clin. Microbiol . 1991; ;29: : 1629– 1634 .
    [Google Scholar]
  5. 5. Rubik   I., , Mirovsky   P., , Dyntarova   H. . Use of lyophilized antigen prepared from continuous cultures of Plasmodium falciparum for serologic diagnosis by the indirect fluorescent antibody test. . Trans R. Soc. Med. Hyg . 1986; ;83: : 6 .
    [Google Scholar]
  6. 6. Woo   PT., , Hauck   L. . The Hematocrit Centrifuge Smear Technique for the detection of mammalian Plasmodium. . Trans. Roy. Soc. Trop. Med. Hyg.   1987; ;81: : 70– 74 .
    [Google Scholar]
  7. 7. Spielman   A. , et al. , Malaria Diagnosis by Direct Observation of Centrifuged Samies of Blood. . Am. J. of Trop. Med. and Hyg.   1988; ;39: 4 : 337– 442 .
    [Google Scholar]
  8. 8. Shute   GT., Godeman   JM. , Identification of Malarial Parasites by Fluorescence Microscopy and Acridine-Orange Staining. . Bull. WHO   1973; ;48: : 591– 596 .
    [Google Scholar]
  9. 9. Rickman   LS., , Long   GW., , Oberst   R. , et al. , Rabid Diagnosis of Malaria by Acridine Orange Staining of Centifuged Parasites. . Lancet . 1989; ; : 68– 71 .
    [Google Scholar]
  10. 10. Bain   BJ. . Blood Cells . , Second Edition. . Oxford and Cambbridge: : Blackwell Science;   1995; :p. 64 .
    [Google Scholar]
  11. 11. Spielman   A., , Perrone   JB. . Rabid Diagnosis of Malaria(letter). . Lancet . 1989; ;1: : 727 .
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.5339/qmj.2002.2.11
Loading
  • Article Type: Research Article
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error