1887
Volume 2000, Issue 2
  • ISSN: 0253-8253
  • E-ISSN: 2227-0426

Abstract

Fifty-six needle liver biopsies from 28 patients with viral hepatitis B (15 cases) and hepatitis C (13 cases) treated with Interferon alpha for a minimum of six months were studied with the aim of determining the role of MGP-positive lymphoid cell infiltrates in the response to treatment with interferon. Besides assessing the MGP-positive cells, the following parameters were assessed and graded: (i) Inflammation in and around the portal tracts, (ii) fibrosis, nodularity and cirrhosis, (iii) piece-meal necrosis (inter-face hepatitis), (iv) lobular activity, (v) stainable iron. Sec-tions were stained with H&E, PAS, PASD, Perl, Masson Trichrome and MGP (Methyl Green Pyronin). All the parameters were graded 1 to 3 except stainable iron which was noted as present or absent.

Fourteen patients (50%) demonstrated histological evidence of a response to treatment. Seven of these showed a very pronounced response with the post treatment liver bi-opsies returning almost to normal. Five of the seven, 27.8% of all MGP stained cases and 71% of all brisk responses, contained ten or more MGP-positive lymphoid cells in the portal tracts.

In contrast, the non-responsive and worsened cases con-tained less than five MGP-positive cells as well as a pre-dominantly small mature lymphocytic infiltrate in the por-tal tracts.

Severity of piece-meal necrosis, lobular activity, portal tract inflammation, the presence or absence of stainable iron, were not of predictive value in the response of viral hepatitis to treatment with interferon alpha.

It appears that MGP-positive lymphoid cells, most prob-ably immune competent activated lymphoid cells, have a positive influence on, and are of predictive value for, the response to treatment of viral hepatitis B and C with inter-feron alpha.

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References

  1. Yoshioka K, Kukumu S, Hayashi H, Shinagawa T, Wakita T, Ishikawa T, Ito Y, Takayanagi M. Anti-Hepatitis C antibodies in patients with chronic non-A non-B hepatitis; Relation to disease progression and effect of interferon alpha. Am J. Gastro-enterol. 1991; 86::14951499.
    [Google Scholar]
  2. Hino K, Sainokami S, Shimoda K, lino S, Wang Y, Okamoto H, Miyakawa Y, Mayumu M. Genotypes and titres of hepatitis C virus for predicting response to interferon in patients with hepatitis C. J. Med. Virol. 1994; 42::299305.
    [Google Scholar]
  3. Yamada G, Takahashi M, Miyamoto R, Tsuji T, Yoshizawa H, Okamoto H. Prediction of interferon effect in chronic hepatitis C by both quantification and genotyping ofHCV-RNA. Dig Dis Sci. 1994; 39::441.
    [Google Scholar]
  4. Koizumi K, Enomotop N, Kurosaki M, Murakami T, Izumi N, Marumo F, Sato C. Diversity of quasispecies in various disease stages of chronic hepatitis C virus infection and its significance in interferon treatment. Hepatology. 1995; 22::3035.
    [Google Scholar]
  5. Stewart WE. The Interferon System. 2nd ed. New York: Springer-Venae 1979.
    [Google Scholar]
  6. Kirchner H. Interferons. A group of multiple lymphokines Springer Semin Immunopathol. 1984; 7::347374.
    [Google Scholar]
  7. Ludwig J. The nomenclature of chronic active hepatitis; an obituary. Gastroenterology. 1993; 105::274278.
    [Google Scholar]
  8. Desmet UJ, Gerber M, Hoofnagle JH, Manns M, Scheur PS. Classification of chronic hepatitis: Diagnosis, Grading and Staging. Hepatology. 1994; 19::15131520.
    [Google Scholar]
  9. Knodell RG, Ishak KG, Black WC, Chen TS, Craig R, Kaplowitz N, Kiernan TW, Wollman J. Formulation and Application of a Numerical Scoring System for Assessing Histological Activity in Asymptomatic Chronic Active Hepatitis. Hepatology. 1981; 1::431435.
    [Google Scholar]
  10. Ishak K, Baptista A, Bianchi L, Callea F, DeGroote J, Gudat F, Denk H, Desmet V, Korb G, MacSween RN, et al., Histological grading and staging of chronic hepatitis. J. Hepatology. 1995; 22::696699.
    [Google Scholar]
  11. Mosnier Jean-Francois, Pham Bach-Nga, Walker Dubois S, Degos F, Erlinger S, et al., Relationship between composition of lymphoid cell infiltrates in the liver and replication status in chronic hepatitis B. An immunolohistochemical study. Am. J. Clin. Pathol. 1996; 106::625633.
    [Google Scholar]
  12. Taniguchi T. Regulation of cytokine gene expression Annu. Rev. Immunol. 1988; 6::439464.
    [Google Scholar]
  13. Goldfeld AE, Maniatis T. Coordinate viral induction of tumour necrosis factor, alpha and interferon beta in human B cells and monocytes. Proc. Natl. Acad. Sci. USA. 1989; 86::14901494.
    [Google Scholar]
  14. Beresini MH, Lempert MJ, Epstein LB. Overlapping polypep-tide induction in human fibroblasts in response to treatment with interferon alpha, interferon gamma, interleukinlalpha, interleukinl-beta and tumour necrosis factor. J. Immunol. 1988; 140::485493.
    [Google Scholar]
  15. Chen L, Mathieu-Mahul D, Bach FH, Dausset J, Bensussan A, Sasporter M. Recombinant interferon alpha can induce rearrangement of T lymphocyte clones in vitro. Pro Natl Acad. Sci. USA. 1986; 83::48874889.
    [Google Scholar]
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