Bacteriophages are viruses that infect and lyse bacteria, the applications of phage techniques in wastewater treatment systems improve effluent and sludge emissions into the environment. The existence of bacteriophages in wastewater of the Soba Stabilisation Station was determined by isolating and identifying methods for their activities against Escherichia coli and Staphylococcus aureus isolated from the anaerobic, facultative and maturation ponds. The general viable count of the bacteria showed an average of 2.0 x 106 cfu/ml. In broth media the affection of the bacteriophage interactions with bacteria showed an increase of bacteriophages with concomitant decrease in bacteria due to culture clearance, where the readings of the turbidity for the first and second infection showed statistical significant light transmission among E. coli phage samples due to placing of sample collections as follows: from the anaerobic and facultative ponds P>0.05, facultative and maturation P<0.05 and anaerobic and maturation P>0.05., whilst the S. aureus phage samples’ light transmission from the anaerobic and facultative P<0.05, facultative and maturation P<0.05 and anaerobic and maturation P>0.05. On solid media, the affection of the bacteriophage was recognised by the phage plaque formation on bacterial cultures. The linear equations of phage densities and distributions according to their wavelength were y = 0.0008x + 0.0303 for E. coli phage and y = -0.0102x + 0.2438 for S. aureus phage.

This study concluded that phages are naturally present, where their hosts are present, and naturally destroyed bacteria which aided recovery from a polluted environment.

Keywords: Bacteriophage/ Escherichia coli/ General viable count / Light transmission/ Linear equations/ Stabilisation Station /Staphylococcus aureus/.


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  • Received: 15 May 2012
  • Accepted: 15 May 2012
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