1887
  1. الرئيسية
  2. A-Z Publications
  3. QScience Connect
  4. المجلد 2013, العدد 1
  5. المقالة
Volume 2013, Issue 1
  • E-ISSN: 2223-506X

ملخص

Unwanted hair is one of the most common medical problems affecting women of reproductive age inducing a lot of psychological stress and threatening their femininity and self-esteem. Old methods of removing unwanted hair include shaving, waxing, chemical depilation, and electrolysis, all of which have temporary results. However laser-assisted hair removal is the most efficient method of long-term hair removal currently available. It is desirable to develop a reduced cost photodynamic therapy (PDT) system whose properties should include high efficiency and low side-effects. Mice skin tissues were used in this study and divided into six groups such as controls, free methylene blue (MB) incubation, liposome methylene blue (MB) incubation, laser without methylene blue (MB), free methylene blue (MB) for 3 and 4 h and laser, liposome methylene blue (MB) for 3 h and laser. Methylene blue (MB) was applied to wax epilated areas. The areas were irradiated with CW He-Ne laser system that emits orange-red light with wavelength 632.8 nm and 10 mW at energy density of 5 J/cm2 for 10 min. The UV-visible absorption spectrum was collected by Cary spectrophotometer. Methylene blue (MB) is selectively absorbed by actively growing hair follicles due to its cationic property. Methylene blue (MB) untreated sections showed that hair follicle and sebaceous gland are intact and there is no change due to the laser exposure. Free methylene blue (MB) sections incubated for 3 h showed that He:Ne laser induced destruction in hair follicles, leaving an intact epidermis. Treated section with free methylene blue (MB) for 4 h showed degeneration and necrosis in hair follicle, leaving an intact epidermis. Liposomal methylene blue (MB) sections incubated for 3 h showed He:Ne laser induced destruction in hair follicles with intradermal leucocytic infiltration. Low power CW He:Ne laser and methylene blue (MB) offered a successful PDT system in selectively damaging hair follicles, leaving an intact epidermis. The current PDT system provides better outcome than hair destruction through laser heat transfer procedures and laser-mediated hair removal, due to complete destruction of hair follicles.

© 2013 Ali, Hashem, Zaher, Korraa, Hamouda, Ali, Al-Saad, licensee Bloomsbury Qatar Foundation Journals.
Loading

جارٍ تحميل قياسات المقالة...

/content/journals/10.5339/connect.2013.16
٢٠١٣-٠٦-٠١
٢٠٢٤-٠٥-١٥
Loading full text...

Full text loading...

/deliver/fulltext/connect/2013/1/connect.2013.16.html?itemId=/content/journals/10.5339/connect.2013.16&mimeType=html&fmt=ahah

References

  1. Wanner M. Laser hair removal. Dermatol Ther. 2005 18::209–216
     [Google الباحث العلمي]
  2. Watts J. Understanding the causes and management of hirsutism. Nurs Times. 2006 102::26–28
     [Google الباحث العلمي]
  3. Al Robaee A, Al-Zolibani A, Al Shobaili H, Aslam M. Update on hirsutism. ActaDermatovenerol Alp Panonica Adriat. 2008 17:3:103–119
     [Google الباحث العلمي]
  4. Liew SH. Laser hair removal: guidelines for management. Am J Clin Dermatol. 2002 3::107–115
     [Google الباحث العلمي]
  5. Dierickx CC. Hair removal by lasers and intense pulsed light sources. Dermatol Clin. 2002 20::135–146
     [Google الباحث العلمي]
  6. Aldraibi MS, Touma DJ, Khachemoune A. Hair removal with the 3-msec alexandrite laser in patients with skin types IV-VI: efficacy, safety, and the role of topical corticosteroids in preventing side effects. J Drugs Dermatol. 2007 6::60–66
     [Google الباحث العلمي]
  7. Rao J, Goldman MP. Prospective, comparative evaluation of three laser systems used individually and in combination for axillary hair removal. Dermatol Surg. 2005 31::1671–1676
     [Google الباحث العلمي]
  8. Davidson D, Rittaca D, Goldman MP. Permanent hyperpigmentation following laser hair removal using the dynamic cooling device. J Drugs Dermatol. 2009 8::68–69
     [Google الباحث العلمي]
  9. Sadick NS, Shaoul J. Hair removal using a combination of conducted radiofrequency and optical energies–an 18-month follow-up. J Cosmet Laser Ther. 2004 6::21–26
     [Google الباحث العلمي]
  10. Haedersdal M, Gøtzsche PC. Laser and photoepilation for unwanted hair growth. Cochrane Database Syst. Rev. 2006 4:CD004684. (1996;35:889–894)
     [Google الباحث العلمي]
  11. Nathans Uebelhoer, Dover Jeffreys. Photodynamic therapy for cosmetic applications. Dermatol. Ther. 2005 18::242–252
     [Google الباحث العلمي]
  12. Peloi LS, Soares RR, Biondo CE, Souza VR, Hioka N, Kimura E. Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue. J Biosci. 2008 33:2:231–237
     [Google الباحث العلمي]
  13. Tardivo JP, Giglio AD, Paschoal LHC, Ito AS, Baptista MS. Treatment of melanoma lesions using methylene blue and RL50 light source. Photodiagn Photodyn Ther. 2004 1::345–346
     [Google الباحث العلمي]
  14. Tardivo JP, Giglio AD, Oliveira CS, Gabrielli DS, Junqueira HC, Tada DB, Severino D, Turchiello RF, Baptista MS. Methylene blue in photodynamic therapy: from basic mechanisms to clinical applications. Photodiagn Photodyn Ther. 2005 2::1–17
     [Google الباحث العلمي]
  15. Wainwright M, Crossley KB. Methylene blue – a therapeutic dye for all seasons. J. Chemother. 2002 14::431–443
     [Google الباحث العلمي]
  16. Calzavara-Pinton PG, Venturini M, Sala R. A comprehensive overview of photodynamic therapy in the treatment of superficial fungal infections of the skin. J Photochem Photobiol B: Biol. 2005 78::1–6
     [Google الباحث العلمي]
  17. Mohamed R. Liposomes Prepartion Methodes. Pak J Pharm Sci. 1996 9:1:65–77
     [Google الباحث العلمي]
  18. Albert M, Lessin MS, Gilchrist BF. Methylene blue: dangerous dye for neonates. J Pediatr Surg. 2003 38::1244–1245
     [Google الباحث العلمي]
  19. Masaharu N, Mitsugu F, Toshio K, Kazuhiko A. Cytogenetic effects of argon laser irradiation on Chinese hamster cells. Radiat Res. 1983 93::598–608
     [Google الباحث العلمي]
  20. Logan ID, McKenna BG, Barnett YA. An investigation of the cytotoxic and mutagenic potential of low intensity laser irradiation in friend erythroleukaemia cells. Mut Res. 1995 347::67–71
     [Google الباحث العلمي]
  21. Bauchinger M. Cytogenetic effects in human lymphocytes as dosimetry systems. In: Eisert WMendelshon M. Biological Dosimetry. Berlin, Heidelberg, New York, Tokyo: Springer-Verlag 1984:15–24
     [Google الباحث العلمي]
  22. Sellheyer K. Mechanisms of laser hair removal: could persistent photoepilationinduce vitiligo or defects in wound repair? Dermatol Surg. 2007 33::1055–1065
     [Google الباحث العلمي]
  23. Dougherty TJ. Photodynamic therapy (PDT) of malignant tumors. Crit Rev Oncol Hematol. 1984 2::83–116
     [Google الباحث العلمي]
  24. Dougherty TJ. Photodynamic therapy. Yearly Review Photochem Photobiol. 1993 55::895–900
     [Google الباحث العلمي]
  25. Grossman MC. Current practice and experience on laser hair removal. In: Arndt KADover JS, Controversies and conversations in cutaneous laser surgery. Chicago, IL: American Medical Association 2002, 267
     [Google الباحث العلمي]
  26. Ruck A, Heckelsmiller K, Akgun N, Beck G, Kunzi-Rapp K, Schick E, Steiner R. Nonlinear dynamics of intracellular methylene blue during light activation of cell cultures. Photochem Photobiol. 1997 66::837–841
     [Google الباحث العلمي]
  27. Schneider MR, Schmidt-Ullrich R, Paus R. Hair follicle as a dynamicminiorgan. Curr Biol. 2009 19::R132–R142
     [Google الباحث العلمي]
  28. Lu Y, Jiao R, Chen X, Zhong J, Ji J, Shen P. Methylene blue-mediated photodynamic therapy induces mitochondria-dependent apoptosis in HeLa cells. J Cell Biochem. 2008 105::1451–1460
     [Google الباحث العلمي]
  29. Kessel D, Luo Y. Mitochondrial photodamage and PDT-induced apoptosis. J Photochem Photobiol B. 1998 42::89–95
     [Google الباحث العلمي]
  30. Chen Y, Zheng W, Li Y, Zhong J, Ji J, Shen P. Apoptosis induced by methylene-blue-mediated photodynamic therapy in melanomas and the involvement of mitochondrial dysfunction revealed by proteomics. Cancer Sci. 2008 99::2019–2027
     [Google الباحث العلمي]
  31. Kleemann D. Experimental studies for photodynamic therapy of malignant tumors of the mouth cavity, larynx and pharynx with the photosensitizer methylene blue. Laryngorhinootologie. 1990 69::437–439
     [Google الباحث العلمي]
  32. McCoy EA, James C. Histological study of hair follicles treated with a 3-msec pulsed rubylaser. Lasers Surg Med. 1999 24::142–150
     [Google الباحث العلمي]
  33. Yao J, Zhang G. Loss of lysosomal integrity caused by the decrease of proton translocation in methylene blue-mediated photosensitization. Biochim Biophys Acta. 1996 1284::35–40
     [Google الباحث العلمي]
  34. Zhang GJ, Yao J. The direct cause of photodamage-induced lysosomal destabilization. Biochim Biophys Acta. 1997 1326::75–82
     [Google الباحث العلمي]
  35. Mellish K, Cox R, Vernon D, Griffiths J, Brown S. In vitro photodynamic activity of a series of methylene blue analogues. Photochem Photobiol. 2002 75::392–397
     [Google الباحث العلمي]
  36. Yu DS, Chang SY, Ma CP. Ultra-structural changes of bladder cancer cells following methylene blue-sensitized photodynamic treatment. Eur Urol. 1991 19::322–326
     [Google الباحث العلمي]
  37. Bangham AD, Hill MW, Miller NGA. Preparation and use of liposomes as models of biological membranes. In: Korn ED, “Methods in Membrane Biology,”. New York: Plenum Press 1974:56–61
     [Google الباحث العلمي]
  38. Zolfaghari PS, Samantha P, Mervyn S. In vivo killing of Staphylococcus aureus using light-activated antimicrobial agent. BMC Microbiol. 2009 9::27–40
     [Google الباحث العلمي]
  39. Zoungrana A, Coulibaly B, Sié A, Walter-Sack I, Mockenhaupt FP, Kouyaté B, Schirmer RH, Klose C, Mansmann U, Meissner P, Müller O. Safety and Efficacy of Methylene Blue Combined with Artesunate or Amodiaquine for Uncomplicated Falciparum Malaria: A Randomized Controlled Trial from Burkina Faso. PLoS ONE www.plosone.org. 2008 3:2:e1630–1-7
     [Google الباحث العلمي]
http://instance.metastore.ingenta.com/content/journals/10.5339/connect.2013.16
Loading
Photodynamic therapy for hair removal
QScience Connect 2013, 16 (٢٠١٣); https://doi.org/10.5339/connect.2013.16
/content/journals/10.5339/connect.2013.16
/content/journals/10.5339/connect.2013.16
Loading

جارٍ تحميل البيانات والوسائط...

  • نوع المستند: Research Article
الموضوعات الرئيسية hair removal, Methylene blue (MB) photosensitizer and He:Ne laser, Photodynamic therapy (PDT) and UV-visible spectroscopy

الأكثر اقتباسًا لهذا الشهر Most Cited RSS feed

هذه الخانة مطلوبة
يُرجى إدخال عنوان بريد إلكتروني صالح
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error