1887
Volume 6 (2025) Number 2
  • EISSN: 2708-0463

Abstract

نُفذتْ الدراسة في مخبر التقانات الحيوية النباتية، التابع للهيئة العامة للتقانة الحيوية، وفي مزارع أبي جرش، كلية الزراعة، جامعة دمشق خلال المدة من عام 2022 حتى عام 2024، بهدف تقييم تأثير الإجهاد الملحي في بعض الصفات المورفولوجية، والبيوكيميائية، لثلاثة أصناف: بطاطا (سفاري، سفنجا، تاروس)، وست سلالات ناتجة من كالس هذه الأصناف ومكاثرة خضرياً بالأنسجة (سفنجا S3، سفنجا S6 ، سفاري SF3، تاروس T1، تاروس T2، تاروس T5)، وتحديد مدى تحملها لهذه الظروف. بعد تقسية الأصناف والسلالات، زُرعت في الحقل، ثم عوملت النباتات بتراكيز مختلفة من ملح كلوريد الصوديوم (0،50،100،150 ميلي مول)، وبمعدل مرة كل ثلاثة أيام، وذلك لمدة شهر. وأظهرت النتائج تباين الأصناف والسلالات المدروسة في استجابتها للإجهاد الملحي، حيث سبّبت الملوحة انخفاضاً في جميع مؤشرات النمو بالمقارنة مع الشاهد. ولُوحظ تَفَوُّق سفنجا على الصنفين سفاري وتاروس في معظم مؤشرات النمو الخضري والجذري. وكان متوسط طول النباتات الأعلى معنوياً لدى الصنف سفاري (55.1 سم)، والسلالة سفنجا S3 (92.27 سم)، ومتوسط المساحة الورقية، فقد كان الصنف سفنجا (21477.66 مم2) وسلالته S6 الأعلى معنوياً (66.26 مم2)، أما عن عدد الجذور، فقد وجدنا تفوق الصنف سفاري (2.11 جذر. نبات-1) والسلالة تاروس T1 (19.24 جذر. نبات-1). كما سبّبت زيادة مستوى الإجهاد الملحي تراجعاً معنوياً في بعض الصفات البيوكيميائية (محتوى الأوراق من اليخضور والكاروتينات)، وازدياداً في بعضها الآخر (البرولين) بالمقارنة مع الشاهد، حيث كان متوسط محتوى الأوراق من البرولين الأعلى معنوياً لدى الصنف سفنجا (1.39 مغ. غ-1) والسلالة تاروس T5 (0.48 مغ. غ-1).

This study was conducted in the plant biotechnology laboratory of the National Commission for Biotechnology, and in Abu Jerash farms, Faculty of Agriculture, University of Damascus during the period from 2022 to 2024. The aim was evaluating the effect of salt stress on Some morphological and biochemical traits of three potato varieties (Safari, Svenga, Tarus) and six potato clones issued from callus culture by micropropagation (Svenga S3, Svenga S6, Safari SF3, Taros T1, Taros T2, Taros T5), also to determine the extent of their tolerance to these conditions. After the acclimatization of these varieties and clones, they were planted in the field. Then the plants were treated with different concentrations of sodium chloride salt (0, 50, 100, 150 mM) at a rate of once every three days for a month. The results showed that the studied varieties and clones were varied in in their response to salt stress, as salinity caused a decrease in all growth indicators compared to the control. It was observed that Svenga was superior to the two Safari and Tarus varieties in most vegetative and root growth indicators. The main plant height was significantly higher for variety Safari (55.1 cm) and clones Svenja S3 (92.27 cm). As for the leaf area, variety Svenga (21477.66 mm2) and its strain S6 was significantly higher (66.26 mm 2). As for the number of roots, we find that variety Safari (2.11 root. Plant -1) and strain Tarus T1 (19.24 root. Plant -1). the increasing level of salt stress caused a significant decline in some biochemical characteristics (leaf chlorophyll content, and carotenoids) and an increase in others (proline) compared with control. The mean leaf content of proline was significantly higher in the variety Svenga (1.39 mg. g-1) and strain Tarus T5 (0.48 mg. g-1).

© 2025 The Author(s), licensee HBKU Press.
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تأثير الإجهاد الملحي في بعض الصفات المورفولوجية والبيوكيميائية لثلاثة أصناف من البطاطا والسلالات المنتخبة من الكالس المجهد
​​​​Arabian Journal of Scientific Research-المجلة العربية للبحث العلمي 6 (2025), 12 (2025); https://doi.org/10.5339/ajsr.2025.12
/content/journals/10.5339/ajsr.2025.12
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  • Article Type: Research Article
Keyword(s): potatoes, salt stress, callus, proline and بطاطا، إجهاد ملحي، كالس، برولين.

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