The application of mesenchymal stem cells differentiated into osteogenic cells is an attractive care modality to enhance the healing process of orthopedics and spinal injuries. Objectives of the in-vitro part of our study are: 1. To isolate and propagate Mesenchymal stem cells ( MSCs) derived from different sources; Bone Marrow (BM), Dental Pulp (DP), and Amniotic Fluid (AF) and 2. Induce their osteogenic differentiation in vitro.

Methods used were the generation and optimization of in-vitro cultures for DP- BM- and AF-MSCs, followed by their osteogenic differentiation by applying three independent induction protocols; osteogenic reagents containing media, bone morphologic protein2 (BMP2), and combined BMP2 and insulin growth factor (IGF).

The results showed that MSCs derived from different sources showed a significant difference both in the proliferation rate and osteogenic differentiation potentials. Whereas, DP-MSCs showed the highest proliferation rate, the AF-MSCs showed the greatest potential to osteogenic differentiation, whereas, BM-MSCs have the least potential toward osteogenic differentiation. In conclusion, the tissue of origin of derived MSCS is greatly influenced by the lineage of differentiation of derived stem cells.


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  • Received: 05 March 2012
  • Accepted: 29 March 2012
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