Volume 1(2020) Number 2

Abstract

تهدف هذه الدارسة إلى تشخيص خميرة وتحديد بعض عوامل الضراوة، وقد أظهر توزيع المرضى المصابين بداء مبيضات الدم أن الفئة العمرية 50-65 سنة سجلت أعلى حالات الإصابة بداء المبيضات عند النساء والرجال المصابين بمرض سرطان الدم بنسبة 50% و37.9% على التوالي مقارنةً بحالات إصابة أقل بداء مبيضات الدم عند الفئة العمرية دون 17 سنة عند النساء والرجال بنسبة8.8% و13.5% على التوالي، في حين كانت حالات الإصابة عند الفئة العمرية 5-8 سنوات مرتفعة، وصلت إلى 18 إصابة بداء مبيضات الفم بنسبة 42% للأطفال المصابين بسرطان الدم مقارنة بالفئة العمرية 8-10 سنوات التي سجّلت حالات إصابة أقل بلغت 9 حالات بنسبة 21%. وقد سُجلت أعلى الإصابات بخميرة ()، إذ بلغت 59 عزلة بين النساء والرجال المصابين بسرطان الدم، في حين كانت الإصابة بخميرة () أقل بلغت عزلة واحدة فقط، بينما بلغت العزلات الفموية من الأطفال المصابين بسرطان الدم لخميرة ()13) عزلة، في حين سُجّل لخميرة () عزلتان فقط. وأظهرت خميرة أعلى تكوين للغشاء الحيوي (Biofilm) فكانت 48 عزلة توزعت بين 21 إنتاجًا قويًا و27 إنتاجًا ضعيفًا للغشاء، أما أقل الخمائر إنتاجًا للغشاء فكانت خميرة () في 6 عزلات توزعت بين 4 ذات إنتاج قوي واثنتين ضعيفتَي الإنتاج للغشاء الحيوي.

This study aimed for isolation and identification of Candida glabrata and identifying some virulence factors. The distribution of patients with candidemia thrush showed that the age group 50-65 years old recorded the highest incidence of candidiasis in female and male with leukemia by 50% and 37.9 % respectively compared to the lowest incidence of candidiasis in the age group under 17 years old in female and male 8.8% and 13.5%, respectively. While the age group between 5-8 years was high, reaching 18 cases of oral candidiasis and 42% of children with leukemia compared with the age group, which was the least, that reached 9 cases, 21%. The highest incidence of was 59 isolates of females and males with leukemia, while was the least which was one isolate. was the most isolated from children with leukemia 13 isolates and was two isolates. showed the highest biofilm formation, 48 isolates were distributed between 21 strong and 27 weak biofilm production. The least biofilm formation isolates were , which reached six isolates and distributed between four strong and two isolates weak.

Loading

Article metrics loading...

/content/journals/10.5339/ajsr.2020.10
2020-11-15
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ajsr/2020/2/ajsr.2020.10.html?itemId=/content/journals/10.5339/ajsr.2020.10&mimeType=html&fmt=ahah

References

  1. Dota KFD, Consolaro MEL, Svidzinski TIE, Bruschi ML. Antifungal activity of Brazilian propolis microparticles against yeasts isolated from vulvovaginal candidiasis. Evidence-Based Complementary and Alternative Medicine. 2011:8.
  2. Mahmoudabadi AZ, Zarrin M, Fard MB. Antifungal susceptibility of Candida species isolated from candiduria. Jundishapur Journal of Microbiology. 2013;6(1):24-28.
  3. Papon, N, Courdavault V, Clastre M, Bennett RJ. Emerging and emerged pathogenic Candida species: Beyond the Candida albicans paradigm. PLOS Pathogens. 2013;9(9):1-4.
  4. Chu JH, Feudtner C, Heydon K, Walsh TJ, Zaoutis TE. Hospitalizations for endemic mycoses: A population-based national study. Clinical Infectious Diseases. 2006;42(6):822-825.
  5. Liu X, Ma Z, Zhang J, Yang L. Antifungal compounds against Candida infections from traditional Chinese medicine. BioMed Research International. 2017:12.
  6. Lipperheide V, Bikandi J, Garcia-Fernandez JF, Quindós G, Pontón J. Colony variation in Candida glabrata isolates from patients with vaginitis. Revista Iberoamericana de Micologia. 2002;19(3):161-164.
  7. Timmermans B, De Las Peñas A, Castaño I, Van Dijck P. Adhesins in Candida glabrata. Journal of Fungi. 2018;4(2):60.
  8. Risan MH. Molecular identification of yeast Candida glabrata from candidemia patients in Iraq. Iraqi Journal of Science. 2016;57(2A):808-813.
  9. Olaniyi OO, Adebowale O. Production and partial purification of beta-mannanase from Aspergillus niger associated with Ilaje Lake, Ondo State, Nigeria. Journal of Bacteriology & Mycology: Open Access. 2017;5(3):281-285.
  10. Collee JG, Fraser AG, Marmion BP, Simmons A. A practical medical microbiology. 14th ed. New York: Churchill Livingstone; 1996.
  11. Vandeppitte J, Engbaek K, Piot P, Heuck CC. Basic laboratory procedures in clinical bacteriology. Geneva: World Health Organization; 1991. V. 85.
  12. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH. Manual of clinical microbiology. 7th ed. Washington, DC: American Society for Microbiology Press; 1999.
  13. Milan EP, Zaror L. Laboratory diagnosis of some types of fungi: Medical mycology. Rio de Janeiro: Editora Guanabara Koogan; 2004.
  14. Zimbro M, Power DA. Difco and BBL manual: Manual of microbiological culture media. 2nd ed. Sparks, MD: Becton, Dickinson and Company; 2009.
  15. Metcalf JA, Gallin JI, Nauseef WM, Root RK. Laboratory manual of neutrophil function. New York: Raven Press; 1986.
  16. Hospenthal DR, Beckius ML, Floyd KL, Horvath LL, Murray CK. Presumptive identification of Candida species other than C. albicans, C. krusei, and C. tropicalis with the chromogenic medium CHROMagar Candida. Annals of Clinical Microbiology and Antimicrobials. 2006;5:1.
  17. Yan LJ, Thangthaeng N, Sumien N, Forster MJ. Serum dihydrolipoamide dehydrogenase is a labile enzyme. Journal of Biochemical and Pharmacological Research. 2013;1(1):30-42.
  18. Saxena N, Maheshwari D, Dadhich D, Singh S. Evaluation of Congo Red Agar for detection of biofilm production by various clinical Candida isolates. Journal of Evolution of Medical and Dental Sciences. 2014;3(59):13234-13238.
  19. SAS. Statistical analysis system: User's guide. Version 9.1. Cary, NC: SAS Institute Inc.; 2012.
  20. Wey SB, Mori M, Pfaller MA, Woolson RF, Wenzel RP. Hospital-acquired candidemia: The attributable mortality and excess length of stay. Archives of Internal Medicine. 1988;148(12):2642-2645.
  21. Zaoutis TE, Argon J, Chu J, Berlin JA, Walsh TJ, Feudtner C. The epidemiology and attributable outcomes of candidemia in adults and children hospitalized in the United States: A propensity analysis. Clinical Infectious Diseases. 2005;41(9):1232-1239.
  22. Schelenz S. Management of candidiasis in the intensive care unit. Journal of Antimicrobial Chemotherapy. 2008;61(Suppl. 1):31-34.
  23. Sonis ST, Fey EG. Oral complications of cancer therapy. Oncology (Williston Park). 2002;16(5):680-686.
  24. Cannon RD, Chaffin WL. Colonization is a crucial factor in oral candidiasis. Journal of Dental Education. 2001;65(8):785-787.
  25. Martins N, Ferreira IC, Barros L, Silva S, Henriques M. Candidiasis: Predisposing factors, prevention, diagnosis and alternative treatment. Mycopathologia. 2014;177(5-6):223-240.
  26. Mohamed BJ, AL-Hussain RA, AL-Thwani AN. Study the inhibitory effect of Lactobacillus acidophilus isolated from yoghurt as probiotics on Candida albicans growth in vitro and in vivo. Iraqi Journal of Biotechnology. 2010;9(2):167-179.
  27. Meurman JH, Siikala E, Richardson M, Rautemaa R. Non-Candida albicans Candida yeasts of the oral cavity. In Méndez-Vilas A, editor. Communicating current research and educational topics and trends in applied microbiology. Badajoz, Spain: Formatex Research Center; 2007. pp. 719-731.
  28. Emmons CW, Binford CH, Utz JP, Chung KJ. Candidiasis. In: Medical mycology. 2nd ed. Philadelphia: Lea and Febiger; 1974. Ch. 14. pp. 167-182.
  29. Calderone RA, Clancy CJ. (2002). Candida and candidiasis. Washington, DC: ASM Press.
  30. Mohammed NA. Detection of Candida spp. and other pathogens responsible for vulvovaginitis in women with contraceptive methods. MSc thesis. College of Science, University of Baghdad, Iraq; 2012.
  31. Manikandan C, Amsath A. Isolation and rapid identification of Candida species from the oral cavity. International Journal of Pure & Applied Bioscience. 2013;1(3):23-27.
  32. Li L, Redding S, Dongari-Bagtzoglou A. Candida glabrata: An emerging oral opportunistic pathogen. Journal of Dental Research. 2007;86(3):204-215.
  33. Wingard JR, Merz WG, Rinaldi MG, Miller CB, Karp JE, Saral R. Association of Torulopsis glabrata infections with fluconazole prophylaxis in neutropenic bone marrow transplant patients. Antimicrobial Agents and Chemotherapy. 1993;37(9):1847-1849.
  34. Fidel PL, Vazquez JA, Sobel JD. Candida glabrata: Review of epidemiology, pathogenesis, and clinical disease with comparison to C. albicans. Clinical Microbiology Reviews. 1999;12(1):80-96.
  35. Ogba OM, Abia-Bassey LN, Epoke J, Mandor BI, Iwatt GD. Characterization of Candida species isolated from cases of lower respiratory tract infection among HIV/AIDS patients in Calabar, Nigeria. World Journal of AIDS. 2013;3(3):201-206.
  36. Mohamed SAA, Al-Ahmadey ZZ. Biofilm formation and antifungal susceptibility of Candida isolates from various clinical specimens. Microbiology Research Journal International. 2013;3(4):590-601.
  37. Mohammed NA. Molecular detection of biofilm encoding genes in Candida albicans isolated from different sources. PhD thesis. College of Science, Baghdad University, Iraq; 2017.
  38. Devi LS, Maheshwari M. Speciation of Candida species isolated from clinical specimens by using Chrom agar and conventional methods. International Journal of Scientific and Research Publications. 2014;4(3):1-5.
  39. Al-Oebady MAH. Isolation and identification of Candida species from vaginal, urine and oral swabs by chromagar Candida. International Journal of Advanced Research. 2015;3(1):948-956.
  40. Hussain RA. A comparison of biological and molecular parameters of some Candida species sensitive and resistant to some antifungal agents. PhD thesis. College of Science, Kufa University, Iraq; 2011.
  41. Akortha EE, Nawaugo VO, Chikwe NO. Antifungal resistance among Candida species from patient with genitourinary tract infection isolated in Benin City, Edo State, Nigeria. African Journal of Microbiology Research. 2009;3(11):694-699.
  42. Sudbery P, Gow N, Berman J. Biodiversity of clinical Candida albicans. Trends in Microbiology. 2004;38(6):869-881.
  43. Maric S, Vranes J. Characteristics and significance of microbial biofilm formation. Periodicum Bilogorum. 2007;109(2):115-121.
  44. Sathiya T, Sheeba MA, Moorthy K, Punitha T, Vinodhini R, Saranya AS. Candida albicans non-albicans species: A study of biofilm production and putative virulence properties. Journal of Harmonized Research in Pharmacy. 2015;4(1):64-75.
  45. Silva S, Henriques M, Oliveira R, Williams D, Azeredo J. In vitro biofilm activity of non-Candida albicans Candida species. Current Microbiology. 2010;61(6):534-540.
  46. Khater ES, Al-Nory MH. Exoenzymes activity and biofilm production in Candida species isolated from various clinical specimens in Benha University Hospital, Egypt. Microbiology Research Journal International. 2014;4(6):654-667.
  47. Mohandas V, Ballal M. Distribution of Candida species in different clinical samples and their virulence: Biofilm formation, proteinase and phospholipase production: A study on hospitalized patients in Southern India. Journal of Global Infectious Diseases. 2011;3(1):4-8.
  48. Dag I, Kiraz N, Oz Y. Evaluation of different detection methods of biofilm formation in clinical Candida isolates. African Journal of Microbiology Research. 2010;4(24):2763-2768.
http://instance.metastore.ingenta.com/content/journals/10.5339/ajsr.2020.10
Loading
/content/journals/10.5339/ajsr.2020.10
Loading

Data & Media loading...

Keyword(s): Candidemia, leukemia, biofilm formation, Baghdad hospitals. and مبيضات الدم، سرطان الدم، تشكيل الغشاء الحيوي، مستشفيات مدينة بغداد.

Most Cited Most Cited RSS feed